Application

DNA Molecular Weight Marker XVII (500 bp ladder) allows accurate sizing of DNA fragments generated by PCR, RT-PCR or restriction digest, following separation on agarose gels. The DNA Molecular Weight Marker XVII can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder), DNA Molecular Weight Marker XIV (100 bp ladder), and DNA Molecular Weight Marker XVI (250 bp ladder) for precise size determination.

General description

Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.Size Range: 0.5 to 5.0 kbp.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Ready-to-use solution, 250µg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).

Preparation Note

Storage conditions (working solution): 2 to 8 °COnce thawed, store at 2 to 8 °C.

Sequence

The mixture contains 10 blunt-ended, double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 500, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, and 5000. The 2000- and 4000-bp banding pattern are three times brighter. Electrophoretic separation of this molecular weight marker results in a regular pattern.Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The fragments exhibit uniform spacing for easier reading, while bands 2000 and 4000 are 3 times brighter for easy orientation.

Unit Definition

50 µg = 1A₂₆₀ unit